Journal: Journal of Cellular and Molecular Medicine

Article Title: HIP/PAP protects against bleomycin‐induced lung injury and inflammation and subsequent fibrosis in mice

doi: 10.1111/jcmm.15334

Figure Lengend Snippet: AdHIP/PAP abates the BLM‐induced upregulation of pro‐inflammatory factors in mouse lungs

Article Snippet: BALF HMGB1, TNF‐α, IL‐1β, and IL‐6 levels were determined by ELISA using commercially available kits (HMGB1 ELISA kit, Chondrex, Redmond, WA, USA; TNF‐α ELISA kit, R&D Systems, Minneapolis, MN, USA; IL‐1β and IL‐6 ELISA kits, eBioscience, San Diego, CA, USA) in accordance with the manufacturer's instructions.

Techniques:

Journal: BMC Infectious Diseases

Article Title: High-mobility group box-1 protein, lipopolysaccharide-binding protein, interleukin-6 and C-reactive protein in children with community acquired infections and bacteraemia: a prospective study

doi: 10.1186/1471-2334-10-28

Figure Lengend Snippet: Levels of HMGB1, LBP, IL-6 and CRP in infected children without SIRS, with sepsis and with severe sepsis.

Article Snippet: HMGB1 levels were measured with a commercially available enzyme-linked immunosorbent assay (HMGB1 ELISA kit; Shino-Test Corporation, Tokyo, Japan).

Techniques: Infection

Journal: BMC Infectious Diseases

Article Title: High-mobility group box-1 protein, lipopolysaccharide-binding protein, interleukin-6 and C-reactive protein in children with community acquired infections and bacteraemia: a prospective study

doi: 10.1186/1471-2334-10-28

Figure Lengend Snippet: Levels of HMGB1, LBP, IL-6 and CRP in children with and without bacteraemia.

Article Snippet: HMGB1 levels were measured with a commercially available enzyme-linked immunosorbent assay (HMGB1 ELISA kit; Shino-Test Corporation, Tokyo, Japan).

Techniques: Infection

Journal: BMC Infectious Diseases

Article Title: High-mobility group box-1 protein, lipopolysaccharide-binding protein, interleukin-6 and C-reactive protein in children with community acquired infections and bacteraemia: a prospective study

doi: 10.1186/1471-2334-10-28

Figure Lengend Snippet: Correlations between HMGB1, LBP, IL-6 and CRP in children with infections

Article Snippet: HMGB1 levels were measured with a commercially available enzyme-linked immunosorbent assay (HMGB1 ELISA kit; Shino-Test Corporation, Tokyo, Japan).

Techniques: Marker

Journal: Stem Cells International

Article Title: HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/ β -Catenin Pathway

doi: 10.1155/2024/2335270

Figure Lengend Snippet: Increased expression and secretion of HMGB1 in TSPCs under high glucose. (a) Relative mRNA levels of HMGB1 in TSPCs by qRT-PCR. (b, c) Western blot analysis of HMGB1 protein expression in TSPCs. (d–f) Western blot analysis of HMGB1 protein levels in the nucleus and cytosol of TSPCs. (g) Immunofluorescence staining for HMGB1 in TSPCs. Scale bar: 50 μ m. (h) ELISA analysis of HMGB1 levels in the medium supernatant of TSPCs under normal glucose or high glucose. ∗ P < 0.05.

Article Snippet: An ELISA kit (Solarbio) was used to detect the levels of HMGB1 in each group according to the manufacturer's protocols.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay

Journal: Stem Cells International

Article Title: HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/ β -Catenin Pathway

doi: 10.1155/2024/2335270

Figure Lengend Snippet: Exogenous HMGB1 promotes the erroneous differentiation of TSPCs. (a) Representative images of ARS staining of TSPCs in BM (A–F) and OIM (G–L) with different concentrations of recombinant HMGB1 (0, 50, and 100 ng/ml) on day 14. (b) Quantification of ARS bound to the calcium nodules in TSPCs. (c–e) Relative mRNA levels of osteogenic genes in TSPCs cultured in BM or OIM with different concentrations of recombinant HMGB1 on day 14 by qRT-PCR. (f–h) Relative mRNA levels of tendon-related genes in TSPCs treated with different concentrations of recombinant HMGB1 by qRT-PCR. A–C, G–I × 1; D–F, J–L × 40. ∗ P < 0.05.

Article Snippet: An ELISA kit (Solarbio) was used to detect the levels of HMGB1 in each group according to the manufacturer's protocols.

Techniques: Staining, Recombinant, Cell Culture, Quantitative RT-PCR

Journal: Stem Cells International

Article Title: HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/ β -Catenin Pathway

doi: 10.1155/2024/2335270

Figure Lengend Snippet: HMGB1 knockdown mitigates high glucose-induced erroneous differentiation of TSPCs. (a) Representative images of ARS staining of TSPCs after HMGB1 knockdown during osteogenic differentiation. (b) Quantification of ARS bound to the calcium nodules in TSPCs. (c–e) Relative mRNA levels of osteogenic genes in TSPCs after HMGB1 knockdown during osteogenic differentiation by qRT-PCR. (f–h) Relative mRNA levels of tendon-related genes in TSPCs after HMGB1 knockdown by qRT-PCR. A–G × 1; F–N × 40. KD, knockdown; vector, negative control vector. ∗ P < 0.05.

Article Snippet: An ELISA kit (Solarbio) was used to detect the levels of HMGB1 in each group according to the manufacturer's protocols.

Techniques: Staining, Quantitative RT-PCR, Plasmid Preparation, Negative Control

Journal: Stem Cells International

Article Title: HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/ β -Catenin Pathway

doi: 10.1155/2024/2335270

Figure Lengend Snippet: Increased HMGB1 activates the RAGE/ β -catenin signaling pathway. (a–d) Western blot analysis of RAGE, β -catenin, and TCF-4 expression in TSPCs during osteogenic induction under normal and high glucose. (e–h) Western blot analysis of RAGE, β -catenin, and TCF-4 expression in TSPCs during osteogenic induction with different concentrations of recombinant HMGB1 (0, 50, and 100 ng/ml). (i–k) Western blot analysis of RAGE and β -catenin expression in TSPCs after HMGB1 knockdown during osteogenic induction. (l–n) Western blot analysis of β -catenin and TCF-4 expression in TSPCs treated with the RAGE inhibitor FPS-ZM1 (10 μ M) during osteogenic induction. KD, knockdown; vector, negative control vector. ∗ P < 0.05.

Article Snippet: An ELISA kit (Solarbio) was used to detect the levels of HMGB1 in each group according to the manufacturer's protocols.

Techniques: Western Blot, Expressing, Recombinant, Plasmid Preparation, Negative Control

Journal: Stem Cells International

Article Title: HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/ β -Catenin Pathway

doi: 10.1155/2024/2335270

Figure Lengend Snippet: Schematic model of the proposed mechanism of erroneous differentiation of TSPCs under high glucose. Under high glucose conditions, increased HMGB1 activates the RAGE/ β -catenin signaling pathway, leading to enhanced osteogenic differentiation of TSPCs and decreased tenogenic differentiation, contributing to the pathogenesis of diabetic tendinopathy.

Article Snippet: An ELISA kit (Solarbio) was used to detect the levels of HMGB1 in each group according to the manufacturer's protocols.

Techniques:

Journal: Journal of Immunology Research

Article Title: Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer

doi: 10.1155/2017/1423683

Figure Lengend Snippet: Plasma levels of HMGB1 during peptide vaccination. Plasma HMGB1 levels of pre- and post-1st cycle vaccination samples of the total 39 cases (a), the 21 serous-type cases (b), and the 18 non-serous-type cases (c) are shown. ns: Not significant.

Article Snippet: The comparisons between plasma HMGB1 levels and other biomarkers were analyzed by t -test and chi-square test.

Techniques: Clinical Proteomics

Journal: Journal of Immunology Research

Article Title: Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer

doi: 10.1155/2017/1423683

Figure Lengend Snippet: The relationship between plasma HMGB1 levels and overall survival (OS) was analyzed by Kaplan-Meier plot analysis. Patients ( n = 39) were divided into high and low plasma HMGB1 groups based on the (a) pre- and (b) postvaccination levels, and the correlation between these subgroups and OS was analyzed. (c) Patients ( n = 39) were also divided into decreased and not-decreased groups based on the changes of plasma HMGB1 between the prevaccination and postvaccination samples, and the relation between these subgroups and OS was plotted.

Article Snippet: The comparisons between plasma HMGB1 levels and other biomarkers were analyzed by t -test and chi-square test.

Techniques: Clinical Proteomics

Journal: Journal of Immunology Research

Article Title: Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer

doi: 10.1155/2017/1423683

Figure Lengend Snippet: HMGB1 and other biomarkers of patients with ovarian cancer treated with peptide vaccination † .

Article Snippet: The comparisons between plasma HMGB1 levels and other biomarkers were analyzed by t -test and chi-square test.

Techniques:

Journal: Journal of Immunology Research

Article Title: Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer

doi: 10.1155/2017/1423683

Figure Lengend Snippet: Correlations between postvaccination plasma HMGB1 levels and postvaccination levels of MDSC frequency, SAA, CRP, or IL-6 were analyzed by scatter plot.

Article Snippet: The comparisons between plasma HMGB1 levels and other biomarkers were analyzed by t -test and chi-square test.

Techniques: Clinical Proteomics

Journal: Journal of Immunology Research

Article Title: Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer

doi: 10.1155/2017/1423683

Figure Lengend Snippet: Comparison of HMGB1 and other biomarkers.

Article Snippet: The comparisons between plasma HMGB1 levels and other biomarkers were analyzed by t -test and chi-square test.

Techniques: Comparison

Journal: Korean Journal of Anesthesiology

Article Title: Damage-associated molecular patterns as a mechanism of sevoflurane-induced neuroinflammation in neonatal rodents

doi: 10.4097/kja.23796

Figure Lengend Snippet: Expression of DAMP molecules in P7 brain tissue and blood serum. (A) Representative IHC image stained with anti-HMGB-1 and anti-galectin-3 in brain tissue. (B) Quantification of positive cell count of HMGB-1 and galectin-3 in brain tissue. (C) Quantification of serum ELISA concentration with anti-HMGB-1, galectin-3, and heparan sulphate in P7 rat blood serum. Values are presented as median (Q1, Q3). *P < 0.05 vs. Con group. Con: control group, DAMPs: damage-associated molecular patterns, ELISA: enzyme-linked immunosorbent assay, HMGB-1: high mobility group box 1, IHC: immunohistochemistry, P7: aged 7 days, Sevo-6h: exposure to sevoflurane for 6 h.

Article Snippet: The levels of HMGB-1, heparan sulphate, and galectin-3 (MyBioSource), as well as interferon-gamma (IFN-γ; Biolegend) in the serum, were measured using commercial ELISA kits following the manufacturer’s instructions.

Techniques: Expressing, Staining, Cell Counting, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control, Immunohistochemistry

Journal: Korean Journal of Anesthesiology

Article Title: Damage-associated molecular patterns as a mechanism of sevoflurane-induced neuroinflammation in neonatal rodents

doi: 10.4097/kja.23796

Figure Lengend Snippet: Effects of ibuprofen on the expression of CD68 and galectin-3 in the P7 rat brain tissue and serum. (A) Representative western blots and quantification of protein levels of CD68 and galectin-3 normalized to GAPDH. (B) Blood serum levels of galectin-3, HMGB-1, and IFN-γ. Values are presented as median (Q1, Q3). * P < 0.05 vs. Con. group, † P < 0.05 vs. Sevo-6h group. CD68: cluster of differentiation 68, Con: control group, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, HMGB-1: high mobility group box 1, IBU: administration of ibuprofen with Sevo-6h, IFN-γ: interferon-gamma, P7: aged 7 days, Sevo-6h: exposure to sevoflurane for 6 h.

Article Snippet: The levels of HMGB-1, heparan sulphate, and galectin-3 (MyBioSource), as well as interferon-gamma (IFN-γ; Biolegend) in the serum, were measured using commercial ELISA kits following the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution’s Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers

doi: 10.3390/ijms25179318

Figure Lengend Snippet: Association of uric acid, HMGB1, IL-6, AIM2, and NLRP3 levels in inflammatory responses. DAMPs measured as HMGB1 and acid uric, inflammasome, measured as AIM2 and NLRP3 and inflammatory related cytokines measured as IL-6 levels were assessed. Results expressed as mean ± SEM. a p < 0.5 vs. SHAM, b p < 0.5 vs. HTK.

Article Snippet: IL-6 and HMGB1: IL-6 and HMGB1 levels in the perfusate were analysed using Labclinics (C. de la Indústria, 54, Gràcia, Barcelona, Spain) assay kits, with references ER0042 and ER0291, respectively.

Techniques: